Alpha-Synuclein

Name: Alpha-Synuclein
SKU: S-1001
Availability: InStock

Product background: Alpha-Synuclein (α-Synuclein) is a presynaptic protein found to be a major component of Parkinson’s Disease (PD) aggregates and is implicated in the pathogenesis of PD and related neurodegenerative disorders.

About the product: Expressed recombinantly in E. coli, human Alpha-Synuclein is purified to our highest standards to ensure batch to batch consistency in both purity and quality.

Applications: The Alpha-Synuclein is sold in 20mM Tris, 100mM NaCl, pH 7.4 to ensure a highly monomeric starting material that could be suited to aggregation studies, seeding experiments, molecular standards, and more.

Catalog ID: S-1001

$300.00 – $500.00

Size

0.5 mg
1.0 mg

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Product Details
- Physical State: White lyophilized powder
- Temperature Storage: -20°C
- Temperature Shipping: Ambient
- Sequence:
  MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA
- Source: Recombinant. A DNA sequence encoding the human alpha-synuclein (1-140) sequence was expressed in E. coli.
- Purity: >95% by SDS-PAGE and Mass Spec.
- Molecular Mass: 14,460 Da theoretical
References

1. Conway, K., et al., (2000) Biochemistry, 39 : 2552
2. Jakes, R., et al., (1994) FEBS Letters, 345 : 27
3. Masliah, E., et al., (2001) Proc. Natl. Acad. Sci., USA, 98 : 12245
4. Ueda, K., et al., (1993) Proc. Natl. Acad. Sci., USA, 90 : 11282
5. Grozdanov, V., et al., (2019) Ann Neurol, 86(4) : 593-606
Publications

Genipin prevents alpha-synuclein aggregation and toxicity by affecting endocytosis, metabolism and lipid storage

Nature Communications (2023) 14 (1), 1918 DOI: 10.1038/s41467-023-37561-2

Rosado-Ramos, Poças, Marques et al

Sigma-2 receptor antagonists rescue neuronal dysfunction induced by Parkinson’s patient brain-derived α-synuclein

Journal of Neuroscience Research; https://doi.org/10.1002/jnr.24782

Limegrover, C., Yurko, R., Izzo, N., et al.

Glucosylceramide Associated with Gaucher Disease Forms Amyloid-like Twisted Ribbon Fibrils That Induce α-Synuclein Aggregation

ACSNANO; https://doi.org/10.1021/acsnano.1c02957

Paul, A., Jacoby, G., Yosef, D., et al.

Sex- and age‐dependent alterations of splenic immune cell profile and NK cell phenotypes and function in C57BL/6J mice

Immunity & Aging; https://doi.org/10.1186/s12979-021-00214-3

Menees, K., Earls, R., Chung, J., et al.

Neuroprotective Effects of Trehalose and Sodium Butyrate on Preformed Fibrillar Form of α-Synuclein-Induced Rat Model of Parkinson’s Disease

American Chemical Society; doi.org/10.1021/acschemneuro.1c00144

Kakoty, V., Sarathlal, C., Dubey, S., et al.

Streamlined alpha-synuclein RT-QuIC assay for various biospecimens in Parkinson’s disease and dementia with Lewy bodies

Acta Neuropathologica Communications; doi: 10.1186/s40478-021-01175-w

Bargar, C., Wang, W., Gunzler, S., et al.

TDP-43–specific Autoantibody Decline in Patients With Amyotrophic Lateral Sclerosis

Neuroimmunology & Neuroinflammation; https://doi.org/10.1212/NXI.0000000000000937

Nielsen, A., Folke, J., Owczarek, S., et al.

Tau-proximity ligation assay reveals extensive previously undetected pathology prior to neurofibrillary tangles in preclinical Alzheimer’s disease

Acta Neuropathologica Communications; https://doi.org/10.1186/s40478-020-01117-y

Vergniory, N., Almpani, E., Silva, A. et al.

Small Molecule Fisetin Modulates Alpha–Synuclein Aggregation

Molecules; doi.org/10.3390/molecules26113353

Ramos, R., Pereira, J., Marques, D., et al.

A rare natural lipid induces neuroglobin expression to prevent amyloid oligomers toxicity and retinal neurodegeneration

bioRxiv; doi.org/10.1101/2021.06.23.449608

Oamen, H., Romero, N., Knuckles, P., et al.

Extracellular α-Synuclein Modulates Iron Metabolism Related Proteins via Endoplasmic Reticulum Stress in MES23.5 Dopaminergic Cells

Neurochem Res; doi.org/10.1007/s11064-021-03292-3

Mi, X., Li, Q., Wen, X., et al.

Decreased AQP4 Expression Aggravates ɑ-Synuclein Pathology in Parkinson’s Disease Mice, Possibly via Impaired Glymphatic Clearance

Springer Link; doi.org/10.1007/s12031-021-01836-4

Cui, H., Wang, W., Zheng, X., et al.

Cerebral dopamine neurotrophic factor reduces α-synuclein aggregation and propagation and alleviates behavioral alterations in vivo

Molecular Therapy; doi.org/10.1016/j.ymthe.2021.04.035

Albert, K., Raymundo, D., Panhelainen, A., et al.

Exogenous Alpha-Synuclein Evoked Parkin Downregulation Promotes Mitochondrial Dysfunction in Neuronal Cells. Implications for Parkinson’s Disease Pathology

Frontiers; doi.org/10.3389/fnagi.2021.591475

Wilkaniec, A., Lenkiewicz, A., Babiec, L., et al.

Skin α-Synuclein Aggregation Seeding Activity as a Novel Biomarker for Parkinson Disease

Jama Neurology; doi:10.1001/jamaneurol.2020.3311

Wang, Z., Becker, K., Donadio, V., et al.

Pharmacologic inhibition of NLRP3 reduces the levels of α-synuclein and protects dopaminergic neurons in a model of Parkinson’s disease

Journal of Neuroinflammation (2023) 20 (1), 147 DOI: 10.1186/s12974-023-02830-w

Amo-Aparicio, Daly, Højen et al

Single-domain antibody–based noninvasive in vivo imaging of α-synuclein or tau pathology

Science Advances (2023) 9 (19), eadf3775 DOI: 10.1126/sciadv.adf3775

Jiang, Lin, Krishnaswamy et al

Molecular determinants of the crosstalk between endosomal microautophagy and chaperone-mediated autophagy

Cell Reports (2023) 42 (12), 113529 DOI: 10.1016/j.celrep.2023.113529

Krause, Kirchner, Stiller et al

The G51D SNCA mutation generates a slowly progressive α-synuclein strain in early-onset Parkinson’s disease

Acta Neuropathol Commun (2023) 11 (1), 72 DOI: 10.1186/s40478-023-01570-5

Lau, Martinez-Valbuena, So et al

Seed Amplification Assay as a Diagnostic Tool in Newly-Diagnosed Parkinson’s Disease

J Parkinsons Dis (2023) 13 (5), 841-844 DOI: 10.3233/JPD-230065

Oftedal, Maple-Grødem, Tysnes et al

Oligomeric and Fibrillar α-Synuclein Display Persistent Dynamics and Compressibility under Controlled Confinement

ACS Chem Neurosci (2023) 14 (21), 3905-3912 DOI: 10.1021/acschemneuro.3c00470

Whitcomb, Warncke

Autoclave treatment fails to completely inactivate DLB alpha-synuclein seeding activity

Biochemistry and Biophysics Reports (2023) 34, 101446 DOI: 10.1016/j.bbrep.2023.101446

Han, Park, Park et al

Nanoplastics exacerbate Parkinson’s disease symptoms in C. elegans and human cells

J Hazard Mater (2024) 465, 133289 DOI: 10.1016/j.jhazmat.2023.133289

Jeong, Park, Lee et al

Distinct tau and alpha-synuclein molecular signatures in Alzheimer’s disease with and without Lewy bodies and Parkinson’s disease with dementia

Acta Neuropathol (2024) 147 (1), 14 DOI: 10.1007/s00401-023-02657-y

van der Gaag, Deshayes, Breve et al

α-Synuclein Pathology in PRKN-Linked Parkinson’s Disease: New Insights from a Blood-Based Seed Amplification Assay

Ann Neurol (2024) 95 (6), 1173-1177 DOI: 10.1002/ana.26917

Kluge, Borsche, Streubel-Gallasch et al

Association of Misfolded α-Synuclein Derived from Neuronal Exosomes in Blood with Parkinson’s Disease Diagnosis and Duration

Journal of Parkinson’s Disease. 2024;14(4):667-679. doi:10.3233/JPD-230390

Schaeffer, Kluge, Schulte et al

Human Microglia–Like Cells Differentiated from Monocytes with GM-CSF and IL-34 Show Phagocytosis of α-Synuclein Aggregates and C/EBPβ-Dependent Proinflammatory Activation

Mol Neurobiol (2024). https://doi.org/10.1007/s12035-024-04289-z

Llaves-López, Micoli, Belmonte-Mateos et al

Fluorescence-Based Monitoring of Early-Stage Aggregation of Amyloid-β, Amylin Peptide, Tau, and α-Synuclein Proteins

ACS Chem Neurosci (2024) 15 (17), 3113-3123 DOI: 10.1021/acschemneuro.4c00097

Li, Awasthi, Bryan et al

Alpha-Synuclein

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References

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