N15 Alpha-Synuclein, Uniformly Labeled
Product background: Alpha-Synuclein (α-Synuclein) is a presynaptic protein found to be a major component of Parkinson’s Disease (PD) aggregates and is implicated in the pathogenesis of PD and related neurodegenerative disorders.
About the product: Expressed recombinantly and isotope labeled in E. coli, human Alpha-Synuclein is purified to our highest standards to ensure batch-to-batch consistency in both purity and quality.
Applications: The N15 labeled Alpha-Synuclein is sold in 20mM Tris, 100mM NaCl, pH 7.4 to ensure a highly monomeric starting material that may be used for NMR structural studies, as a molecular weight standard, a biomarker, and more.
$450.00 – $750.00
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Product Details
- Physical State: White lyophilized powder
- Temperature Storage: -20°C
- Temperature Shipping: Ambient
- Sequence:
MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA
- Source: Recombinant. A DNA sequence encoding the human alpha-synuclein sequence was expressed in E. coli, with N15 as the nitrogen source.
- Purity: >95% by SDS-PAGE and Mass Spec.
- Molecular Mass: 14,624 Da theoretical
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References
1. Conway, K., et al., (2000) Biochemistry, 39 : 2552
2. Jakes, R., et al., (1994) FEBS Letters, 345 : 27
3. Masliah, E., et al., (2001) Proc. Natl. Acad. Sci., USA, 98 : 12245
4. Ueda, K., et al., (1993) Proc. Natl. Acad. Sci., USA, 90 : 11282
5. Grozdanov, V., et al., (2019) Ann Neurol, 86(4) : 593-606 -
Publications
Nat Commun (2024) 15 (1), 3658 DOI: 10.1038/s41467-024-47980-4
Frontiers in Neuroscience; doi: 10.3389/fnins.2021.643391
Journal of Proteome Research; 10.1021/acs.jproteome.7b00660.
